Data files

8-week-old male Cx3cr1CreERT2/+; Abca1flox/flox; Abcg1flox/flox experimental animals (KO) and Cx3cr1CreERT2/+ controls (Ctrl) were used for EAE experiments. Myelin oligodendrocyte glycoprotein (MOG)-EAE was induced in mice purchased from Charles River Laboratories by subcutaneous immunization with 200 mg of MOG peptide 35–55 (MOG35–55) in complete Freund’s adjuvant (Mycobacterium tuberculosis at 3.75 mg ml−1; BD) and intraperitoneal injection twice with 500 ng of pertussis toxin (Sigma). Animals ...

Primary mouse microglia were purified from early postnatal brains, seeded on coated slides in defined medium, and exposed to a myelin plus IFN-γ activation regimen. After fixation and permeabilization, a 500-gene MERFISH panel was applied to spatially resolve lipid-metabolism and inflammatory transcripts at single-cell resolution, enabling unbiased identification of distinct microglial states across the culture.

Remyelination can occur naturally in demyelinating lesions, but often fails in human demyelinating diseases such as multiple sclerosis (MS). The function of the innate immune system is essential for the regenerative response, but how exactly microglia and macrophages clear myelin debris after injury and tailor a specific regenerative response is unclear. Here, we asked whether pro-inflammatory microglial/macrophage activation is required for this process. We established a novel toxin-based spinal ...