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CNS nuclei were isolated from frozen thalamic specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

Brains were homogenized, stained, microglia were FACS-purified, RNA was isolated, cDNA was produced and sequencing libraries were prepared.

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen CNS tissue specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

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