Data files

Cortex and corpus callosum were dissected from every two animals (3-month-old) and pooled into one sample for single-nuclei RNA sequencing using 10x genomics Chromium 3’ assay. Each genotype has 2 replicates (in total n=4 animals).

Brain hemispheres were dissected from 6-month-old animals and subjected to single-nuclei RNA sequencing using 10x genomics Chromium 3’ assay. In total 4 genotypes were included for the experiment (WT, Cnp-/-, 5xFAD, Cnp-/-x5xFAD), each genotype has n=2 replicates.

Microglia cells from 6-month-old mouse brain hemispheres were isolated using the MACS sorting system and subjected to 50 bp single-end mRNA sequencing. Each genotype has n=4 replicates, in total 4 genotypes were included for experiment (WT, Cnp-/-, 5xFAD, Cnp-/-x5xFAD).