Data files

CNS nuclei were isolated from frozen thalamic specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

Brains were homogenized, stained, microglia were FACS-purified, RNA was isolated, cDNA was produced and sequencing libraries were prepared.

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen CNS tissue specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

Frozen autopsy brain tissue from fourteen multiple-sclerosis donors and three neurologically healthy controls was sectioned at –20 °C into 80–100 µm slices, and an 8 mm punch (~25 mg) of white matter or matched control tissue was collected from each region of interest . Nuclei were liberated with the 10x Genomics Chromium Nuclei Isolation Kit, using an extended lysis–debris-removal protocol tailored to lipid-rich white matter; the final suspension was stained with propidium iodide and counted ...

In this study, our experimental design focused on the detailed analysis of lesion areas in the brain tissues of patients with multiple sclerosis (MS). First, we collected brain tissue samples from 14 MS patients. Using specialized staining techniques, we accurately identified the locations of MS lesions within the brain tissues. Subsequently, we used a brain puncher to extract the brain tissue regions containing lesions from each sample. These precisely cut samples were then analyzed on the ...