Data files

CNS nuclei were isolated from frozen thalamic specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

Brains were homogenized, stained, microglia were FACS-purified, RNA was isolated, cDNA was produced and sequencing libraries were prepared.

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen thalamic specimen, FACS purified, and analyzed with snRNA-seq

CNS nuclei were isolated from frozen CNS tissue specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with snRNA-seq

Microglia are the key immune cells involved in virtually all diseases of the central nervous system (CNS). Here, we generated a novel large-scale single-cell RNA-sequencing-derived taxonomy through the analysis of > 1 million CNS cells enriched for myeloid cells across > 15 different pathologies and conditions. Disease-associated human microglia, together with CNS-associated macrophages and monocytes, were differentiated iteratively into 27 superclusters and 192 clusters. This top-down ...

CNS nuclei were isolated from frozen specimen, stained with anti-NeuN and anti-Olig2, FACS purified (DAPI+NeuN-Olig2-), and analyzed with single nucleus RNA-seq.