Data files

RNA-seq profiling of iPSC‑derived microglia, including wild‑type controls and mutant lines for Slc37a2 and TREM2, at different timepoints, with biological replicates.

CD8+ T cells from PBMCs of the MS twin cohort (n = 12; 6 healthy twins, 6 twins with SCNI, and 12 twins with MS; 193,771 cells) and the validation cohort (n = 17; 5 individuals with IIH and 12 individuals with MS; 89,859 cells) were isolated via FACS and processed using the 10X manufacturer's protocol to generate single-cell transcriptome and TCR libraries. The corresponding TCR sequences are included within the metadata of the uploaded objects. (Author states that fastq raw data files from humans ...

Inflammation in the central nervous system (CNS) can impair the function of neuronal mitochondria and contributes to axon degeneration in the common neuroinflammatory disease multiple sclerosis (MS). Here we combine cell type-specific mitochondrial proteomics with in vivo biosensor imaging to dissect how inflammation alters the molecular composition and functional capacity of neuronal mitochondria. Neuroinflammatory lesions in the mouse spinal cord cause widespread and persisting axonal ATP ...

Spatial transcriptomics of the intact and injured mouse cerebral cortex at 3 days post injury (dpi).

Single cell transcriptomics of all cells of the intact and injured mouse cerebral cortex at 3 and 5 days post injury (dpi), in control condition and upon inhibition of the Tlr1/2 and Cxcr3 pathways.

Astrocyte heterogeneity has been well explored, but our understanding of white matter (WM) astrocytes and their distinctions from grey matter (GM) astrocytes remains limited. Here, we compared astrocytes from cortical GM and WM/corpus callosum (WM/CC) using single cell RNA-sequencing (scRNA-seq) and spatial transcriptomics of the murine forebrain. The comparison revealed similarities, but also significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks ...

Astrocyte heterogeneity has been extensively studied, but our understanding of white matter (WM) astrocytes and their distinctions from grey matter (GM) astrocytes remains limited. We directly compared astrocytes from cortical GM, WM, and the subependymal zone using scRNA-seq and spatial transcriptomics of murine forebrain sections. The comparison revealed significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM astrocytes. Furthermore, ...