Shiny-based web visualisation: Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter Version 1
Astrocyte heterogeneity has been well explored, but our understanding of white matter (WM) astrocytes and their distinctions from grey matter (GM) astrocytes remains limited. Here, we compared astrocytes from cortical GM and WM/corpus callosum (WM/CC) using single cell RNA-sequencing (scRNA-seq) and spatial transcriptomics of the murine forebrain. The comparison revealed similarities, but also significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM astrocytes with molecular properties also shared with human WM astrocytes. When comparing murine astrocytes from two different WM regions, cortex and cerebellum, we found that they exhibit distinct, region-specific molecular properties, with the cerebellum lacking for example a specific cluster of WM astrocytes expressing progenitor and proliferation genes. Functional experiments confirmed astrocyte proliferation in WM/CC, but not cerebellar WM, suggesting that the WM/CC may be a source of continued astrogenesis.
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Created: 4th Aug 2026 at 11:39
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Version 1 (earliest) Created 4th Aug 2026 at 11:39 by Camilla Giudici
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Astrocyte heterogeneity has been well explored, but our understanding of white matter (WM) astrocytes and their distinctions from gray matter (GM) astrocytes remains limited. Here, we compared astrocytes from cortical GM and WM/corpus callosum (WM/CC) using single-cell RNA sequencing and spatial transcriptomics of the murine forebrain. The comparison revealed similarities but also significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM ...
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