Expression profiling: Single-cell multiome (scRNA-seq + scATAC-seq), Bulk ATAC-seq, Bulk RNA-seq, ChIP-seq, Cut&Run, Methyl-HiC (mouse)

https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

Neuronal loss in acute or chronic brain disease could be treated by neuronal replacement strategies, such as transplantation of young neurons, if they integrate properly into the existing networks. Here we explore the cell-based and molecular mechanisms causing hyperinnervation and overshoot loss of input connectivity as well as the newly discovered hurdle in output connectivity, the lack of myelination. Candidate as well as unbiased approaches are pursued to identify the critical checkpoints and ...

Direct neuronal reprogramming is a promising approach to regenerate neurons from local glial cells. However, mechanisms of epigenome remodeling and co-factors facilitating this process are unclear. In this study, we combined single-cell multiomics with genome-wide profiling of three-dimensional nuclear architecture and DNA methylation in mouse astrocyte-to-neuron reprogramming mediated by Neurogenin2 (Ngn2) and its phosphorylation-resistant form (PmutNgn2), respectively. We show that Ngn2 drives ...

Single cell multiome (scRNA-seeq + scATAC-seq), bulk ATAC-seq, bulk RNA-seq, ChIP-seq, Cut&Run and Methyl-HiC was performed on primary mouse astrocytes (2 days post induction) transduced with doxycycline-inducible lentiviral constructs expressing either GFP, Ngn2 and PmutNgn2 respectively. Since the constructs also expressed GFP, the astrocytes were FACS sorted based on GFP expression. To profile the effect of Yy1 on direct neuronal reprogramming. scRNA-seq and bulk ATAC-seq was performed on ...