Direct neuronal reprogramming of mouse astrocytes is associated with multiscale epigenome remodeling and requires Yy1 Version 1
Single cell multiome (scRNA-seeq + scATAC-seq), bulk ATAC-seq, bulk RNA-seq, ChIP-seq, Cut&Run and Methyl-HiC was performed on primary mouse astrocytes (2 days post induction) transduced with doxycycline-inducible lentiviral constructs expressing either GFP, Ngn2 and PmutNgn2 respectively. Since the constructs also expressed GFP, the astrocytes were FACS sorted based on GFP expression. To profile the effect of Yy1 on direct neuronal reprogramming. scRNA-seq and bulk ATAC-seq was performed on Yy1 KO primary mouse astrocytes (2 days post induction) transduced with a doxycycline-inducible lentiviral construct expressing Ngn2.
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Created: 4th Aug 2026 at 08:19
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Version 1 (earliest) Created 4th Aug 2026 at 08:19 by Camilla Giudici
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Web page: Not specified
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Direct neuronal reprogramming is a promising approach to regenerate neurons from local glial cells. However, mechanisms of epigenome remodeling and co-factors facilitating this process are unclear. In this study, we combined single-cell multiomics with genome-wide profiling of three-dimensional nuclear architecture and DNA methylation in mouse astrocyte-to-neuron reprogramming mediated by Neurogenin2 (Ngn2) and its phosphorylation-resistant form (PmutNgn2), respectively. We show that Ngn2 drives ...
Submitter: Camilla Giudici
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: C05 - Glial checkpoints for neuronal circuitry ...
