Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex

https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

Traumatic brain injuries (TBIs) cause primary tissue damage and activate glial cells to build glial border, thereby delineating the injured brain parenchyma from the healthy surrounding tissue. However, this necessary primary reaction of glial cells often persists. This long-lasting glial activation results in prolonged and exacerbated neuroinflammation that prevents tissue restoration. We propose that a cross-regulatory network between innate immunity pathways and EV cargo defines an important ...

Spatial transcriptomics of the intact and injured mouse cerebral cortex at 3 days post injury (dpi).

Single cell transcriptomics of all cells of the intact and injured mouse cerebral cortex at 3 and 5 days post injury (dpi), in control condition and upon inhibition of the Tlr1/2 and Cxcr3 pathways.