Data files

Genome binding/occupancy profiling by high throughput sequencing

Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing

scRNA-seq and scATAC-seq of the somsatosensory area of the E14.5 mouse cortex. Additionally, massively parallel reporter assay (MPRA) and MethylHiC from isolated cell populations, namely neuronal stem cells (NSC), intermediate progenitors (IPC), projection neurons (PN), obtained from the somatosensory area of the E14.5 mouse cortex. Functional validation of Neurog2 using Hi-C, RNA-seq and NOME-seq in GFP and Neurog2 in vivo overexpression via in utero electroporation.

Single cell multiome (scRNA-seeq + scATAC-seq), bulk ATAC-seq, bulk RNA-seq, ChIP-seq, Cut&Run and Methyl-HiC was performed on primary mouse astrocytes (2 days post induction) transduced with doxycycline-inducible lentiviral constructs expressing either GFP, Ngn2 and PmutNgn2 respectively. Since the constructs also expressed GFP, the astrocytes were FACS sorted based on GFP expression. To profile the effect of Yy1 on direct neuronal reprogramming. scRNA-seq and bulk ATAC-seq was performed on ...