Federico Bleckwedel

I am a Ph.D. student in the group of Dr. Laura Zelarayan at the Institute of Pharmacology and Toxicology (UMG). I am working to decipher the transcription factors (TF) involved in the transition to heart failure using a murine model. For that, I am analyzing Single-Cell RNAseq and RNAseq data to find the most critical genes implied in regulating this pathological remodeling. Afterward, an inference based on the transcriptome signature would suggest TF targets. Those targets would be confirmed via Chip-MS and Chip-Seq. Finally, a CRISPR dCas9 approach would be proposed to recover the heart gene homeostasis. The development of this project would bring us closer to finding a safe and powerful gene-therapy approach for treating myocardial infarction.