Data files

In order to investigate the effect of antibiotics on the CNS, we performed a RNA-Seq analysis on spinal cord-derived microglia and total spinal cord from wild type LEW/Crl rats (Rattus norvegicus) after 7 days of intratracheal treatment with PBS, neomycin (NEO) or vancomycin (VANCO).

In order to investigate the effect of antibiotics on the expression profile of T cells, RNA-Seq analysis was performed on MBP-reactive T cells sorted from the lungs of LEW/Crl rats (Rattus norvegicus) intratracheally pre-treated for 7 days with PBS or NEO. Analyis was performed on day 0 and day 1 after intratacheal immunization with MBP.

We isolated single nuclei from white matter post-mortem tissue from 5 non-neurological control samples (Ctrl) and post-mortem tissue from 4 progressive Multiple sclerosis (MS) patients. We got a total of 20 samples, which in the MS samples included different non-lesioned (normal appearing white matter) and lesioned (active lesions, chronic active lesions, chronic inactive lesions and remyelinating lesions) based on pathology, to address the cellular and functional differences between the two ...

Brain hemispheres were dissected from 6-month-old animals and subjected to single-nuclei RNA sequencing using 10x genomics Chromium 3’ assay. In total 4 genotypes were included for the experiment (WT, Cnp-/-, 5xFAD, Cnp-/-x5xFAD), each genotype has n=2 replicates.

Total of 16 mice brains with raw data for 50,212 single cells and processed data for 37,089 single cells