Integrative single-cell analysis of transcriptional and epigenetic states in the human adult brain

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https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

Erythropoietin (EPO) is a cytokine that serves, independent of hematopoiesis, signalling functions within the brain, where EPO and its receptor are upregulated upon injury. The underlying mechanisms, however, have remained obscure. We aim to understand the roles of EPO/EPOR signalling as checkpoints of brain homeostasis and thereby specifically predict effects of recombinant EPO as a neuroprotective drug. To this end, we will explore the induction/expression of EPO and EPOR at the single cell ...

Amyloid-β (Aβ) is thought to be neuronally derived in Alzheimer’s disease (AD). However, transcripts of amyloid precursor protein (APP) and amyloidogenic enzymes are equally abundant in oligodendrocytes (OLs). By cell-type-specific deletion of Bace1 in a humanized knock-in AD model, APPNLGF, we demonstrate that OLs and neurons contribute to Aβ plaque burden. For rapid plaque seeding, excitatory projection neurons must provide a threshold level of Aβ. Ultimately, our findings are relevant for AD ...