Spatial map of microglia states across CNS diseases

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https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

Childhood-onset white matter diseases including multiple sclerosis and monogenetic leukoencephalopathies, are characterized by neuroinflammatory tissue damage. Here, we propose that CD8+ T cell phenotypes within lesions serve as critical immune checkpoints for tissue damage and regeneration. Our objective is to define CD8+ T cell-driven disease and regenerative pathways by utilizing a combination of multifluorescent immunohistochemistry, spatial transcriptomics and multimodal MR imaging in patients ...

Single-cell studies have revealed substantial microglial diversity in development, homeostasis and disease. However, a framework enabling comparison and stratification of microglial states across contexts is needed. Here we generated an atlas of myeloid cell states by single-cell RNA sequencing more than one million central nervous system cells from more than 30 physiological and pathological conditions. This atlas enables us to establish a comprehensive taxonomy of myeloid cell states across ...