TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions

https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

B lymphocytes have been more recently recognized to possess a dual role in MS pathogenesis. On the one hand, B cells promote inflammation of the central nervous system and MS progression, while on the other hand, B-lymphoid inhibition of pathogenic T cell functions ameliorates MS severity and facilitates recovery from acute MS bouts. To understand the functional dichotomy, we aim at a comprehensive phenotyping of individual B cell subpopulations followed by their transcriptional profiling to ...

To investigate TFEB-dependent expression in BCR-stimulated and resting B cells, CRISPR/Cas9 knockout cell lines were established and subjected to RNA sequencing analysis

To investigate TFEB-dependent mRNA expression in murine B cells, FACS-sorted germinal center and non-germinal center B lymphocytes of B-cell-specific conditional TFEB KO mice and control littermates were subjected to RNA sequencing analysis