Expression profiling: scRNA-seq (mouse)

https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

The medical burden of stroke extends beyond the brain injury itself and is largely determined by chronic comorbidities that develop secondarily. We hypothesized that these comorbidities might share a common immunological cause, yet chronic effects post-stroke on systemic immunity are underexplored. Here, we identify myeloid innate immune memory as a cause of remote organ dysfunction after stroke. Single-cell sequencing revealed persistent pro-inflammatory changes in monocytes/macrophages in ...

A single-cell RNA experiment was performed on cardiac interstitial cells from mice one month after stroke or control conditions

A single-cell RNA experiment was performed on bone marrow myeloid progenitor and mature cells chronically after stroke

A single-cell RNA experiment was performed on blood and heart myeloid cells from BM-depleted recipient mice that were transplanted with bone marrow cells from stroke or control donors.

Three independent mouse single-cell RNA experiments were performed on myeloid cells from different peripheral organs (blood, spleen, heart, lung, liver) and bone marrow, chronically after stroke and control conditions.