Innate immune memory after brain injury drives inflammatory cardiac dysfunction

https://rdp.sfb274.de/ (Research Data Platform) https://gepris.dfg.de/gepris/projekt/408885537 (DFG Gepris entry) The central nervous system (CNS) is a terminally differentiated tissue, where any insult carries a heightened risk - yet the tissue response to these insults is variable and can range from irreversible destruction to almost complete recovery. The rules that instruct these divergent outcomes are still unknown. The aim of this CRC is therefore to understand the biology of the multicellular ...

CUT&tag sequencing of mouse BM monocytes and HSPC-enriched myeloid cells from mice one month after stroke or control conditions was performed

Bulk mRNA sequencing of human heart paraffin embeded samples from stroke patients and control patients

Bulk ATAC sequencing of mouse HSPC-enriched BM cells treated with recombinant IL-1b or vehicle

Mouse single-nuclei ATAC experiment performed on myeloid cells from bone marrow chronically after stroke and control conditions. An additional group of bone marrow cells from stroke mice treated with neutralizing antibodies against IL-1b was also included (stroke-treated group).

A single-cell RNA experiment was performed on cardiac interstitial cells from mice one month after stroke or control conditions

A single-cell RNA experiment was performed on bone marrow myeloid progenitor and mature cells chronically after stroke

A single-cell RNA experiment was performed on blood and heart myeloid cells from BM-depleted recipient mice that were transplanted with bone marrow cells from stroke or control donors.

Three independent mouse single-cell RNA experiments were performed on myeloid cells from different peripheral organs (blood, spleen, heart, lung, liver) and bone marrow, chronically after stroke and control conditions.